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Role of type I myosins in receptor-mediated endocytosis in yeast

M I Geli1, H Riezman

  • 1Department of Biochemistry, Biozentrum, University of Basel, Klingelbergstrasse 70, Basel, Switzerland.

Science (New York, N.Y.)
|April 26, 1996
PubMed

Insights

Yeast type I myosins (MYO3 and MYO5) are crucial for cell growth. Deleting these genes severely impairs receptor-mediated endocytosis, specifically the internalization step, highlighting their essential role in this cellular process.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Type I myosins are implicated in actin-dependent membrane dynamics.
  • In vivo evidence for their specific roles in cellular processes has been limited.

Purpose of the Study:

  • To investigate the in vivo function of yeast type I myosins (encoded by MYO3 and MYO5).
  • To determine the specific cellular processes dependent on type I myosin activity.

Main Methods:

  • Gene deletion of MYO3 and MYO5 in yeast.
  • Complementation of a double deletion mutant with a temperature-sensitive MYO5 allele (myo5-1).
  • Analysis of membrane traffic, including endocytosis and secretion.

Main Results:

  • Deletion of MYO3 and MYO5 genes almost abolished yeast growth.
  • The myo5-1 mutant exhibited a significant defect in the internalization step of receptor-mediated endocytosis.
  • The secretory pathway appeared unaffected in the mutant.

Conclusions:

  • Yeast type I myosin activity is essential for a budding event during endocytosis.
  • Type I myosins are not required for all aspects of membrane traffic, such as secretion.

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