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Related Experiment Videos

Prostate cancer: normal prostate from human and hamster

T G Pretlow

    National Cancer Institute Monograph
    |December 1, 1978
    PubMed
    Summary

    The term "prostatic acid phosphatase" is ambiguous. Current assays lack specificity, failing to distinguish prostatic acid phosphatase from other acid phosphatases in various cell types.

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    Area of Science:

    • Biochemistry
    • Urology
    • Cell Biology

    Background:

    • The term "prostatic acid phosphatase" (PAP) is widely used but ambiguously defined.
    • PAP is associated with prostate diseases and exhibits properties like inhibitor sensitivity, substrate specificity, antigenic characteristics, and presence in prostate tissues/secretions.
    • Existing evidence supporting the term "prostatic acid phosphatase" is largely indirect.

    Purpose of the Study:

    • To investigate the specificity of substrates and inhibitors used for measuring prostatic acid phosphatase.
    • To determine if current methods can accurately distinguish acid phosphatase activity originating from prostatic epithelial cells versus other cell types.

    Main Methods:

    • Purification of specific cell types from prostate and other tissues.
    • Utilizing purified cells as sources for studying enzymes derived from particular cell types.
    • Evaluating the effectiveness of claimed specific substrates and inhibitors for prostatic acid phosphatase measurement.

    Main Results:

    • Acid phosphatase activity was measured using various substrates and one inhibitor.
    • None of the tested substrates or inhibitors demonstrated sufficient specificity.
    • The assays could not reliably differentiate acid phosphatase activity from prostatic epithelial cells from that of other purified cell types.

    Conclusions:

    • Current substrates and inhibitors used for measuring prostatic acid phosphatase lack the necessary specificity.
    • Distinguishing prostatic acid phosphatase activity from other cellular acid phosphatases remains a challenge with existing methods.

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