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Evidence for a new step in telomere maintenance

R J Wellinger¹, K Ethier, P Labrecque

¹Department of Microbiology, Faculty of Medicine, Université de Sherbrooke, Quebec, Canada.

|May 3, 1996

Summary

Telomeres, the protective caps on chromosomes, can interact via G-rich tails. A novel mechanism involving DNA strand degradation appears to generate these tails, aiding telomere maintenance.

Area of Science:

Molecular Biology
Genetics
Cell Biology

Background:

Telomeres are G-rich DNA sequences at chromosome ends.
Telomerase generates G-rich overhangs (G tails) at telomeres.
Telomere interactions have been observed in yeast.

Purpose of the Study:

To investigate the mechanism of telomere-telomere interactions.
To explore the role of G tails in these interactions.
To identify novel pathways in telomere maintenance.

Main Methods:

In vitro generation of molecules mimicking telomere replication intermediates.
Analysis of telomere-telomere interactions in yeast.
In vivo studies in yeast cells lacking telomerase.

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Main Results:

In vitro generated molecules with G tails showed telomere-telomere interactions.
These interactions mimicked those observed in yeast.
G tails were generated in vivo even without telomerase activity.

Conclusions:

Telomere-telomere interactions are facilitated by G tails.
Cell cycle-regulated degradation of the C1-3A strand is a novel telomere maintenance step.
This degradation generates substrates for telomerase and telomere-binding proteins.