The G-banding pattern of chromosomes in Ehrlich Lettŕe ascites tumor cells

The trypsin digestion-Giemsa staining technique was used to produce distinctive banding patterns in chromosomes of Ehrlich Lettŕe ascites tumor cells derived from a frozen culture originally begun from the ELD line. The chromosome numbers most frequently encountered in cells were 44 and 45. Four large marker chromosomes were identified in the karyotype. One of these was a large submetacentric chromosome, the banding pattern of which indicated its probable origin was from a pair of telocentric chromosomes. Using banding patterns and lengths, the chromosomes were classified into four groups--marker, paired, unpaired, and minute chromosomes.