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Developmentally imprinted genes as markers for bladder tumor progression

M J Cooper1, M Fischer, D Komitowski

  • 1Department of Medicine, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106-4937, USA.

The Journal of Urology
|June 1, 1996
PubMed
Summary

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H19 gene expression increases with bladder tumor grade, suggesting it may drive bladder cancer progression. This oncodevelopmental marker is found in advanced tumors and carcinoma in situ.

Area of Science:

  • Oncology
  • Developmental Biology
  • Genetics

Background:

  • Developmentally imprinted genes like H19 and insulin-like growth factor-II (IGF-II) are crucial for embryogenesis.
  • These genes have been linked to the development of childhood embryonal tumors.
  • H19 expression is observed in the human fetal bladder.

Purpose of the Study:

  • To evaluate H19 expression in bladder carcinomas using in situ hybridization.
  • To correlate H19 expression with tumor grade in 35 bladder carcinomas.
  • To assess the expression of H19, IGF-II, IGF-I, and the type I IGF receptor in bladder cell lines for potential gene transfer studies.

Main Methods:

  • In situ hybridization was employed to analyze H19 expression in bladder tumor tissues.
  • Northern analysis was utilized to determine the expression levels of H19, IGF-II, IGF-I, and the type I IGF receptor in bladder cell lines.

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Main Results:

  • H19 expression was significantly higher in advanced stage bladder tumors (grade II and III) and carcinoma in situ compared to grade I tumors (p = 0.004).
  • Normal bladder mucosa cells did not express H19.
  • Three of eleven bladder cell lines (HT-1376, HT-1197, 5637) showed high H19 mRNA levels, and these cell lines, along with J82, expressed IGF-II. All cell lines expressed the type I IGF receptor, but not IGF-I.

Conclusions:

  • H19 functions as an oncodevelopmental marker for bladder tumor progression.
  • The findings suggest H19 may possess oncogenic properties in the context of bladder cancer.