Related Experiment Video
Updated: Aug 10, 2026

Methods to Discover Alternative Promoter Usage and Transcriptional Regulation of Murine Bcrp1
Published on: May 27, 2016
Identification of a promoter region in the rat prion protein gene
K Saeki1, Y Matsumoto, Y Matsumoto
1Department of Molecular Immunology, Faculty of Agriculture, University, of Tokyo, Japan.
Abstract:
We have demonstrated the presence of a rat prion protein (RaPrP) gene promoter upstream of multiple initiation sites. A 0.1-kb fragment upstream of the 5'-untranslated region contains specific DNA motifs characteristic of promoter elements including an AP-1 binding site, an inverted CCAAT motif and three inverted Sp-1 binding sites. This fragment directs transcription of a luciferase reporter gene in pheochromocytoma cells (PC12) and rat glioma cells (C6), suggesting that it contains the promoter for the RaPrP gene. To more precisely localize the transcription regulatory elements in this region, a series of 5'-deletion mutants were generated. Deletion analysis showed that an inverted CCAAt and adjoining Sp-1 binding sequences may play an important role in transcription of the RaPrP gene.
Related Concept Videos
RNA Polymerase II Accessory Proteins
The Eukaryotic Promoter Region
Reporter Genes
Commonly used reporter...
Ribosome Profiling
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique helps...
Transcription Initiation
The promoters and enhancers and their accessory proteins allow tight regulation of...

