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Complete exon structure of the ALL1 gene
D Rasio1, S A Schichman, M Negrini
1Kimmel Cancer Institute, Jefferson Medical College of Thomas Jefferson University, Philadelphia, Pennsylvania 19107, USA.
Cancer Research
|April 15, 1996
Summary
The ALL1 gene, implicated in leukemias, has its complete genomic structure defined. Understanding its 36 exons and 90 kb span aids research into gene fusions and potential roles in solid tumors.
Area of Science:
- Genetics
- Molecular Biology
- Oncology
Background:
- The ALL1 gene is frequently rearranged in acute leukemias.
- Gene fusions involving ALL1 are observed in acute lymphoblastic leukemia (ALL) and acute myeloid leukemia (AML).
Purpose of the Study:
- To elucidate the complete genomic structure of the ALL1 gene.
- To investigate the role of ALL1 in leukemogenesis and solid malignancies.
Main Methods:
- Detailed genomic mapping of the ALL1 gene.
- Analysis of gene structure, including exon-intron boundaries.
Main Results:
- The ALL1 gene spans approximately 90 kb on chromosome band 11q23.
- It comprises 36 exons with sizes ranging from 65 bp to 4249 bp.
- Intronic sequences flanking exon boundaries were identified.
Conclusions:
- The defined genomic structure facilitates the study of ALL1 rearrangements in leukemia.
- This information is crucial for investigating potential point mutations in solid tumors with 11q23 loss of heterozygosity.