Related Experiment Video
Updated: Aug 5, 2026

Gene-targeted Random Mutagenesis to Select Heterochromatin-destabilizing Proteasome Mutants in Fission Yeast
Published on: May 15, 2018
An additional copy of the adenylate cyclase-encoding gene relieves developmental defects produced by a mutation in a
G Loubradou1, J Bégueret, B Turcq
1Laboratoire de Génétique Moléculaire des Champignons Filamenteux, UPR CNRS 9026, Université de Bordeaux II, France.
Abstract:
To identify cellular functions involved in vegetative incompatibility in filamentous fungi, we have initiated the cloning of Podospora anserina (Pa) mod genes. These genes interfere with the lethal reaction triggered by interaction between incompatible het genes. A gene (Pa AC) has been cloned by complementation of developmental defects caused by a mutation in the mod-D gene. This gene encodes a protein of 2145 amino acids (aa)that exhibits strong similarities with many adenylate cyclases (AC). About 65% aa identity has been found between the sequence of the polypeptide encoded by this Pa AC gene and the AC of Neurospora crassa. The organization of peptidic domains in the polypeptide encoded by Pa AC is closely related to that of Saccharomyces cerevisiae CYR1. Restriction-fragment-length polymorphism (RFLP) and genetic analysis have shown that Pa AC and mod-D are distinct genes.
Related Concept Videos
Monohybrid Crosses
In-vitro Mutagenesis
Genome Copying Errors
Exon Recombination
Exon shuffling follows “splice frame rules.” Each exon has three reading...
Transgenic Plants
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...
Anaphase Promoting Complex

