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Construction of an alkaline phosphatase fusion-generating transposon, mTn10phoA
1Department of Microbiology and Immunology, University of Michigan Medical School, Ann Arbor 48109-0620, USA. mcclain@umich.edu.
Gene
|April 17, 1996
Abstract:
We constructed a derivative of the mini-transposon mTn10 that generates translational fusions to the phoA gene from Escherichia coli and carries the KmR determinant from Tn5. This new transposon, mTn10phoA, is carried on a mobilizable plasmid with both selectable and counterselectable markers. The plasmid carrying mTn10phoA was introduced into Legionella pneumophila. Southern hybridization analysis indicated that the mTn10phoA insertions were randomly distributed.