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Related Experiment Videos

Alpha 2-adrenergic agonists increase cellular lactate efflux

W Lockette1, K Kirkland, S Farrow

  • 1Division of Endocrinology, Wayne State University School of Medicine, Detroit, MI 48201, USA.

Hypertension (Dallas, Tex. : 1979)
|May 1, 1996
PubMed
Summary
This summary is machine-generated.

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Genetic variations in alpha 2-adrenergic receptors may protect against cold by boosting heat generation through increased lactate production. This finding offers insights into evolutionary adaptations and metabolic regulation.

Area of Science:

  • Physiology
  • Genetics
  • Metabolic Research

Background:

  • Genetic polymorphisms of the alpha 2-adrenergic receptor are linked to hyperinsulinemia, diabetes, and hypertension in Black populations.
  • The evolutionary advantage of these polymorphisms remains unclear.
  • Vascular alpha 2-adrenergic receptors are known to mediate cold-induced vasoconstriction and play a role in thermoregulation.

Purpose of the Study:

  • To investigate the role of vascular alpha 2-adrenergic receptors in metabolic heat generation.
  • To determine if alpha 2-adrenergic agonists influence thermogenesis via aerobic lactate production.
  • To examine lactate metabolism in vascular smooth muscle cells of genetically hypertensive rats.

Main Methods:

  • Measured metabolic heat production using aerobic lactate efflux in HT29 cells expressing human vascular alpha 2-adrenergic receptors.

Related Experiment Videos

  • Administered alpha 2-adrenergic agonists (epinephrine and clonidine) to assess their effect on lactate production.
  • Analyzed lactate production in vascular smooth muscle cells from stroke-prone spontaneously hypertensive rats (SHRSP) and Wistar-Kyoto (WKY) rats, with and without glucose.

    • Main Results:

    • Alpha 2-adrenergic agonists (epinephrine and clonidine) significantly increased lactate efflux in HT29 cells.
    • Insulin also stimulated lactate production in these cells.
    • Vascular smooth muscle cells from SHRSP exhibited significantly higher lactate efflux compared to WKY cells, independent of glucose uptake.

    Conclusions:

    • Vascular alpha 2-adrenergic receptors contribute to thermogenesis through aerobic lactate production.
    • Abnormalities in vascular smooth muscle lactate metabolism are present in genetically hypertensive rats.
    • Balanced polymorphisms of the alpha 2-adrenergic receptor may confer protection against cold stress by enhancing thermogenesis.