Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Functional domains in the Mig1 repressor

J Ostling1, M Carlberg, H Ronne

  • 1Ludwig Institute for Cancer Research, Uppsala, Sweden.

Molecular and Cellular Biology
|March 1, 1996
PubMed
Summary

The Mig1 protein in yeast regulates glucose repression via its C-terminal effector domain. The Snf1 protein kinase negatively controls Mig1 activity through internal elements, impacting gene transcription.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prevalence of prurigo nodularis in Sweden.

Journal of the European Academy of Dermatology and Venereology : JEADV·2024
Same author

Taurine-like Immunoreactivity in the Motor Nerve Net of the Jellyfish Cyanea capillata.

The Biological bulletin·2017
Same author

Component-resolved in vitro diagnosis of carrot allergy in three different regions of Europe.

Allergy·2012
Same author

Requirement for isoprenoid-dependent posttranslational modifications in the cell-cycle progression of human breast-cancer cells.

International journal of oncology·2011
Same author

Presence of 3,4-dihydroxyphenylalanine and 3,4,5-trihydroxyphenylalanine in a coelenterate nervous system: Possible tyrosinase-mediated accumulation.

Neurochemistry international·2010
Same author

Enhancement of hazelnut extract for IgE testing by recombinant allergen spiking.

Allergy·2007

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Transcriptional Regulation

Background:

  • Mig1 is a zinc finger protein in Saccharomyces cerevisiae responsible for glucose repression.
  • It shares homology with mammalian transcription factors like Krox/Egr, Wilms' tumor, and Sp1.
  • Mig1 binds to GC-rich DNA motifs similar to GC boxes.

Purpose of the Study:

  • To identify functional domains within the Mig1 protein.
  • To investigate the regulatory mechanisms controlling Mig1 activity.
  • To elucidate the role of the Snf1 protein kinase in Mig1 regulation.

Main Methods:

  • Deletion mapping to identify functional domains of Mig1.
  • Construction and analysis of a Mig1-VP16 hybrid activator.
  • Assessing reporter gene expression and cell growth on various carbon sources.

Main Results:

  • A C-terminal domain of 24 amino acids mediates Mig1-dependent gene repression.
  • This effector domain contains leucine-proline dipeptide repeats.
  • Internal elements within Mig1 inhibit its activity in the absence of glucose.
  • The Snf1 protein kinase negatively regulates Mig1 activity via these internal elements.

Conclusions:

  • The C-terminal domain is crucial for Mig1's effector function in gene repression.
  • Mig1 activity is modulated by internal regulatory elements and the Snf1 kinase.
  • Understanding these regulatory mechanisms provides insight into glucose metabolism control in yeast.

Related Experiment Videos