[The first pregnancy in the Czech Republic achieved by oocyte fertilization using ICSI--intracytoplasmic sperm...

ICSI--an intracytoplasmic sperm injection can fertilize an oocyte in vitro with one sperm, using micromanipulation. ICSI was done on an inverted Diaphot 300 microscope with Hoffmann modulation contrast and Narishige micromanipulators. Between March and May 1995 23 ICSI cycles were performed. Of 166 oocytes (7.2 per cycle) 22% were degenerated or immature and 6% damaged during ICSI. 45% of the injected eggs were fertilized. Embryos were transferred in 65% couples. Three pregnancies resulted (20%/ET), all are viable. ICSI is suitable in cases of severe oligospermia, asthenospermia and teratospermia, immunology infertility and low cleavage rate of oocytes in the first IVF cycle.