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L-selectin in patients with common variable immunodeficiency (CVID): a comparative study with normal individuals
J G Zhang1, L Morgan, G P Spickett
1Department of Immunology, University of Newcastle upon Tyne, UK.
Clinical and Experimental Immunology
|May 1, 1996
Summary
Common Variable Immunodeficiency (CVID) patients show reduced L-selectin on lymphocytes, with increased soluble L-selectin in plasma. This may impact immune cell function and trafficking in CVID.
Area of Science:
- Immunology
- Cell Adhesion Molecules
Background:
- L-selectin mediates leukocyte adhesion to high endothelial venules.
- Soluble L-selectin is detectable in biological fluids and elevated in various diseases.
Purpose of the Study:
- To investigate L-selectin expression on leukocytes in CVID patients.
- To measure soluble L-selectin levels in CVID patient plasma.
Main Methods:
- Flow cytometry to assess L-selectin expression on peripheral blood leukocytes.
- ELISA to quantify soluble L-selectin in plasma.
Main Results:
- Significant loss of L-selectin expression on CVID B cells and CD4+ T cells (CD45RO+).
- Normal L-selectin expression on non-lymphoid leukocytes.
- Significantly elevated soluble L-selectin levels in CVID plasma compared to healthy controls.
Conclusions:
- Reduced lymphocyte L-selectin in CVID may result from aberrant lymphocyte activation.
- Down-regulation of L-selectin contributes to increased soluble L-selectin levels.
- Altered lymphocyte trafficking is suggested in CVID patients.