Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Two distinct roles for Ras in a developmentally regulated cell migration

T Lee1, L Feig, D J Montell

  • 1Department of Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.

Development (Cambridge, England)
|February 1, 1996
PubMed
Summary

Ras protein activity is crucial for cell migration during Drosophila oogenesis. Reduced Ras activity initiates migration, while sustained Ras activity is required for ongoing cell movement, impacting border cell migration dynamics.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microbial diversity in biofilms on water distribution pipes of different materials.

Water science and technology : a journal of the International Association on Water Pollution Research·2010
Same author

Outbreak of brucellosis in domestic elk in Korea.

Zoonoses and public health·2009
Same author

Acute enterovirus infection followed by myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) and viral persistence.

Journal of clinical pathology·2009
Same author

Daily rhythms and sex differences in vasoactive intestinal polypeptide, VIPR2 receptor and arginine vasopressin mRNA in the suprachiasmatic nucleus of a diurnal rodent, Arvicanthis niloticus.

The European journal of neuroscience·2009
Same author

Measurement of bottom versus charm as a function of transverse momentum with electron-hadron correlations in p + p collisions at square root of s = 200 GeV.

Physical review letters·2009
Same author

IGF-I, leptin and active ghrelin levels in very low birth weight infants during the first 8 weeks of life.

Acta paediatrica (Oslo, Norway : 1992)·2009

Area of Science:

  • Developmental Biology
  • Cell Biology
  • Genetics

Background:

  • Receptor tyrosine kinases (RTKs) regulate cell movement.
  • Ras proteins are key downstream effectors of RTKs, influencing cell motility.
  • Border cells in Drosophila oogenesis provide a model system to study cell migration.

Purpose of the Study:

  • To investigate the role of Ras in border cell migration during Drosophila oogenesis.
  • To determine how Ras activity influences the initiation and execution of border cell migration.
  • To identify downstream effectors of Ras involved in this process.

Main Methods:

  • Expression of dominant-negative and constitutively active Ras in Drosophila border cells.
  • Analysis of border cell migration initiation and progression.

Related Experiment Videos

  • Genetic manipulation involving mutations in slbo1 (Drosophila C/EBP) and Ras activity.
  • Examination of downstream Ras effectors, Raf and Ral.

    • Main Results:

    • Dominant-negative Ras inhibited migration when expressed during migration but promoted premature initiation when expressed before migration.
    • Constitutively active Ras delayed migration initiation.
    • Reduced Ras activity rescued the migration defect in slbo1 mutants.
    • Raf activity was dispensable, but Ral activity was required for migration initiation.

    Conclusions:

    • Ras activity has dual roles in border cell migration: promoting initiation and being essential for sustained migration.
    • Ras regulates border cell migration through a Raf-independent pathway, involving Ral.
    • These findings elucidate the dynamic regulation of cell migration by Ras signaling.