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Related Experiment Videos

Human native soluble CD40L is a biologically active trimer, processed inside microsomes

F Pietravalle1, S Lecoanet-Henchoz, H Blasey

  • 1Glaxo Institute for Molecular Biology, Geneva, Switzerland.

The Journal of Biological Chemistry
|March 15, 1996
PubMed
Summary

Soluble CD40 ligand (CD40L), a protein involved in immune responses, is shed from T cells. This soluble form, when combined with interleukin-4, promotes B cell proliferation.

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Area of Science:

  • Immunology
  • Molecular Biology
  • Cell Biology

Background:

  • CD40 ligand (CD40L) is crucial for B cell activation, mediating proliferation, adhesion, and immunoglobulin class switching upon binding to its receptor CD40.
  • CD40L is expressed on activated T cells and other immune cells.

Purpose of the Study:

  • To identify and characterize soluble cleavage products of human CD40L.
  • To investigate the processing and function of soluble CD40L.

Main Methods:

  • Stimulation of human T cell clones and collection of supernatant.
  • Subcellular fractionation to analyze CD40L processing.
  • Biochemical characterization of soluble CD40L (e.g., trimeric structure).
  • In vitro assays to assess the effect of soluble CD40L on B cell proliferation, often in combination with interleukin-4.

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Main Results:

  • Soluble CD40L cleavage products were identified in the supernatant of stimulated T cells.
  • Transmembrane CD40L processing occurs within microsomes and is dependent on cell stimulation.
  • Native soluble human CD40L exists as a trimer.
  • Soluble CD40L, with interleukin-4, effectively induces B cell proliferation.

Conclusions:

  • CD40L undergoes stimulation-dependent cleavage, releasing soluble forms.
  • Soluble CD40L retains biological activity, capable of inducing B cell proliferation.
  • Understanding CD40L processing and function is important for immune regulation research.