Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Translational initiation factor eIF-4E. A link between cardiac load and protein synthesis

H Wada1, C T Ivester, B A Carabello

  • 1Department of Medicine, Gazes Cardiac Research Institute and Veterans Administration Medical Center, Charleston, South Carolina 29401-5799, USA.

The Journal of Biological Chemistry
|April 5, 1996
PubMed
Summary

Cardiac load increases protein synthesis via eIF-4E phosphorylation. Mechanical stress, not just any load, triggers this response, impacting cardiac anabolism.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Achievement of Target Gain Larger than Unity in an Inertial Fusion Experiment.

Physical review letters·2024
Same author

Lawson Criterion for Ignition Exceeded in an Inertial Fusion Experiment.

Physical review letters·2022
Same author

Thirty-Day Mortality for Proximal Femoral Fractures Treated at a U.K. Elective Center with a Site-Streaming Policy During the COVID-19 Pandemic.

JB & JS open access·2021
Same author

Risk factors for non-communicable diseases related to obesity among first- and second-generation Bangladeshi migrants living in north-east or south-east England.

International journal of obesity (2005)·2021
Same author

Observations of pressure anisotropy effects within semi-collisional magnetized plasma bubbles.

Nature communications·2021
Same author

Report of a Delphi exercise to inform the design of a research programme on screening for thoracic aortic disease.

Trials·2020

Area of Science:

  • Cardiology
  • Molecular Biology
  • Biochemistry

Background:

  • Cardiac protein synthesis is crucial for adaptation to mechanical stress.
  • The precise mechanisms linking cardiac load to protein synthesis regulation remain incompletely understood.

Purpose of the Study:

  • To investigate the role of eukaryotic initiation factor 4E (eIF-4E) phosphorylation in mediating the relationship between cardiac load and protein synthesis.
  • To differentiate the effects of mechanical stress versus other stimuli on eIF-4E phosphorylation in cardiomyocytes.

Main Methods:

  • Measuring eIF-4E phosphorylation in adult feline cardiocytes subjected to electrical stimulation and varying conditions (e.g., presence of 2,3-butanedione monoxime).
  • Assessing eIF-4E phosphorylation in canine models with acute hemodynamic overload (pressure and volume).

Related Experiment Videos

  • Utilizing pharmacological agents like insulin and phorbol 12-myristate 13-acetate to modulate eIF-4E phosphorylation.

    • Main Results:

    • Electrical stimulation of cardiocytes significantly increased eIF-4E phosphorylation, an effect blocked by inhibiting actin-myosin cross-bridge cycling.
    • Insulin and phorbol 12-myristate 13-acetate also increased eIF-4E phosphorylation, but independently of mechanical tension.
    • In vivo, left ventricular pressure overload elevated eIF-4E phosphorylation, while acute volume overload did not.

    Conclusions:

    • eIF-4E phosphorylation is a key signaling mechanism coupling mechanical cardiac load to accelerated protein synthesis.
    • The response is specific to pressure-induced mechanical stress, explaining differential anabolic responses to various overload types.
    • This finding provides insight into the molecular basis of cardiac adaptation and maladaptation.