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Related Experiment Videos

CD39 is an ecto-(Ca2+,Mg2+)-apyrase

T F Wang1, G Guidotti

  • 1Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.

The Journal of Biological Chemistry
|April 26, 1996
PubMed
Summary
This summary is machine-generated.

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CD39 is an ecto-apyrase enzyme found on Epstein Barr virus-transformed B cells. This enzyme plays a role in immune cell function and nucleotide metabolism.

Area of Science:

  • Immunology
  • Molecular Biology
  • Biochemistry

Background:

  • CD39 is a molecule identified on activated lymphoid cells, specifically Epstein Barr virus (EBV)-transformed B cells.
  • Previous research identified CD39 as a surface marker without fully characterizing its enzymatic function.

Purpose of the Study:

  • To investigate the enzymatic activity associated with CD39 on immune cells.
  • To determine if CD39 possesses ecto-apyrase activity.
  • To elucidate the role of CD39 in nucleotide metabolism and immune cell function.

Main Methods:

  • Assessing ecto-(Ca2+,Mg2+)-apyrase activity on EBV-transformed B cells and comparing it with B and T lymphomas.
  • Analyzing the amino acid sequence homology of CD39 with known nucleotide triphosphatases.

Related Experiment Videos

  • Expressing CD39 in COS-7 cells to confirm its enzymatic function.

    • Main Results:

    • EBV-transformed B cells exhibit ecto-apyrase activity, which is absent in B and T lymphomas.
    • CD39's amino acid sequence shows significant homology to nucleotide triphosphatases.
    • Expression of CD39 in COS-7 cells confirmed its ecto-apyrase activity.

    Conclusions:

    • CD39 is confirmed to be an ecto-apyrase, an enzyme involved in nucleotide hydrolysis.
    • The findings suggest CD39 plays a role in immune cell regulation and nucleotide metabolism.
    • This study establishes a direct link between CD39 expression and enzymatic function in immune cells.