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Genomic structure and promoter analysis of the human obese gene
1Molecular and Cellular Endocrinology Branch and Phoenix Epidemiology and Clinical Research Branch, NIDDK, National Institutes of Health, Bethesda, Maryland 20892, USA.
The Journal of Biological Chemistry
|February 23, 1996
Abstract:
The human gene encoding the homolog of the mouse obese (ob) gene was isolated and partially characterized. The human ob gene consists of three exons and two introns and spans about 18 kilobase pairs (kb), encoding a 3.5-kb cDNA. A 3-kb 5'-flanking region of the gene was cloned and transient transfection assay with luciferase reporter confirmed the promoter activity in differentiated F442-A adipocytes. Potential regulatory elements are discussed in this report.