Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ultraviolet induced mutations in Aspergillus wentii

S Sinha, S L Chakrabarty

    Acta Microbiologica Polonica
    |January 1, 1978
    PubMed
    Summary

    UV radiation created new Aspergillus wentii mutants with altered conidia color, growth needs, and amylase activity. Arginine-deficient mutants exhibited enhanced amylase production, a key finding for enzyme research.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Purification and characterization of extracellular β-glucosidase from Myceliophthora thermophila.

    World journal of microbiology & biotechnology·2014
    Same author

    Ground state proton radioactivity from 121Pr: when was this exotic nuclear decay mode first discovered?

    Physical review letters·2005
    Same author

    An efficient synthesis of aryloxyphenyl cyclopropyl methanones: a new class of anti-mycobacterial agents.

    Bioorganic & medicinal chemistry letters·2005
    Same author

    Painful gynecomastia following traumatic spinal cord injury.

    Clinical neurology and neurosurgery·2005
    Same author

    Prognostic factors in patients presenting with severe neurological forms of Wilson's disease.

    QJM : monthly journal of the Association of Physicians·2005
    Same author

    A 5-year audit of haemodialysis access.

    International journal of clinical practice·2005

    Area of Science:

    • Microbiology
    • Industrial Biotechnology
    • Enzyme Engineering

    Background:

    • Aspergillus wentii is a fungus with industrial applications, particularly in enzyme production.
    • UV radiation is a known mutagen used to generate genetic diversity in microorganisms.
    • Understanding enzyme activity in fungal mutants can lead to improved industrial processes.

    Purpose of the Study:

    • To generate and characterize new mutants of Aspergillus wentii using UV mutagenesis.
    • To investigate the impact of mutagenesis on fungal characteristics like conidia color and growth factor requirements.
    • To assess the amylase activity of the obtained mutants, focusing on arginine-deficient strains.

    Main Methods:

    • Irradiation of Aspergillus wentii (IMI 17295) and its nutritional mutant strains with UV rays.
    • Isolation and characterization of new mutant strains.
    • Evaluation of conidia color, growth factor requirements, and amylase activity.
    • Comparative analysis of enzyme activity between parent strains and mutants.

    Main Results:

    • UV mutagenesis successfully generated distinct Aspergillus wentii mutants.
    • Mutants exhibited variations in conidia pigmentation and specific nutritional requirements.
    • Significant differences in amylase activity were observed among the mutant strains.
    • Arginine-deficient mutants demonstrated notably higher amylase activity compared to parent strains.

    Conclusions:

    • UV mutagenesis is effective in creating diverse Aspergillus wentii mutants with altered phenotypes.
    • Nutritional deficiencies, specifically arginine deficiency, can correlate with enhanced amylase production.
    • These findings offer potential for developing improved fungal strains for industrial enzyme applications.

    Related Experiment Videos