Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved method for selecting RNA-binding activities in vivo

D E Fouts1, D W Celander

  • 1Department of Microbiology, University of Illinois, Urbana 61801, USA.

Nucleic Acids Research
|April 15, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic modifications to temperate Enterococcus faecalis phage Ef11 that abolish the establishment of lysogeny and sensitivity to repressor, and increase host range and productivity of lytic infection.

Microbiology (Reading, England)·2013
Same author

Probing RNA structures with hydroxyl radicals.

Current protocols in nucleic acid chemistry·2008
Same author

Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus faecalis.

Science (New York, N.Y.)·2003
Same author

Complete genome sequence and comparative analysis of the metabolically versatile Pseudomonas putida KT2440.

Environmental microbiology·2003
Same author

Novel RNA-binding properties of Pop3p support a role for eukaryotic RNase P protein subunits in substrate recognition.

The Journal of biological chemistry·2001
Same author

Pseudomonas syringae Hrp type III secretion system and effector proteins.

Proceedings of the National Academy of Sciences of the United States of America·2000

Researchers developed a faster method to create RNA challenge phages, which are tools for studying RNA-protein interactions inside cells. This new technique uses a single bacterial recombination step, simplifying phage construction.

Area of Science:

  • Molecular Biology
  • Virology
  • Biochemistry

Background:

  • RNA challenge phages are engineered bacteriophages used to study in vivo RNA-protein interactions.
  • Conventional methods for constructing these phages involve multiple homologous recombination steps in bacteria.
  • A streamlined method is needed to improve the efficiency of RNA challenge phage generation.

Purpose of the Study:

  • To develop an improved and simplified method for constructing RNA challenge phages.
  • To demonstrate the utility of the new method by creating a specific phage derivative.

Main Methods:

  • The study describes a novel in vivo construction strategy for RNA challenge phages.
  • This method relies on a single homologous recombination event within bacterial cells.

Related Experiment Videos

  • The improved technique was used to generate a P22R17 derivative.

    • Main Results:

    • The new method successfully constructs RNA challenge phages using a single homologous recombination reaction.
    • A derivative of P22R17 was efficiently generated using the improved technique.
    • The constructed phage is capable of lysogenic development in the presence of specific coat proteins.

    Conclusions:

    • The improved method significantly simplifies the construction of RNA challenge phages.
    • This advancement facilitates the study of RNA-protein interactions in vivo.
    • The new technique offers a more accessible approach for generating specialized bacteriophages.