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Helical fold prediction for the cyclin box

¹Protein Machine Group, Department of Molecular Biology, DNAX Research Institute, Palo Alto, California 94304-1104, USA.

|January 1, 1996

Summary

Researchers predict the structure of the cyclin box domain, crucial for cell cycle regulation and transcription. Evolutionary sequence data reveals a fold composed of tandem, packed helical modules.

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Area of Science:

Biochemistry and Molecular Biology
Structural Biology
Genetics

Background:

Eukaryotic cell cycle progression depends on cyclins, which activate cell cycle kinases.
Cyclin homologs and related structures are involved in transcription regulation and tumor suppression.
The cyclin box is a conserved structural motif within cyclins.

Purpose of the Study:

To predict the secondary, supersecondary, and tertiary structure of the minimal cyclin box domain.
To utilize evolutionary information from multiple sequence alignments for structural prediction.

Main Methods:

Comparative sequence analysis using multiple sequence alignments.
Computational techniques for predicting protein structure based on evolutionary data.

Main Results:

A prediction for the canonical secondary, supersecondary, and tertiary fold of the cyclin box domain was generated.
The predicted fold consists of a tandem set of closely packed helical modules.

Conclusions:

The study provides a structural model for the minimal cyclin box domain.
The predicted helical structure offers insights into the functional roles of cyclins in cell cycle and transcription.

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