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Related Experiment Videos

Expression of hemoglobin E in newborn

N Sae-ung1, G Fucharoen, S Fucharoen

  • 1Department of Clinical Microscopy, Faculty of Associated Medical Sciences, Khon Kaen University, Thailand.

The Southeast Asian Journal of Tropical Medicine and Public Health
|January 1, 1995
PubMed
Summary

Hemoglobin E (HbE) levels are typically underestimated in newborns. This study found that HbE expression in cord blood correlates with the number of beta E globin genes, aiding neonatal screening.

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Area of Science:

  • Genetics
  • Hematology
  • Molecular Biology

Background:

  • Hemoglobin E (HbE) is an abnormal hemoglobin variant.
  • It results from a specific point mutation in the beta-globin gene.
  • HbE levels are often underrepresented in newborns compared to adults.

Purpose of the Study:

  • To investigate the relationship between HbE genotype and HbE levels in cord blood.
  • To provide data for improving neonatal screening programs for HbE.

Main Methods:

  • Analysis of 145 cord blood specimens.
  • Starch gel electrophoresis for HbE detection.
  • Microcolumn chromatography for quantifying HbE.
  • Polymerase chain reaction (PCR) for beta E globin gene zygosity determination.

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Main Results:

  • HbE levels were measured in heterozygotes (3.17 +/- 1.79%), homozygotes (8.55 +/- 2.52%), and normal newborns (0.48 +/- 0.22%).
  • A clear correlation was observed between the number of beta E globin gene copies and HbE expression.

Conclusions:

  • Newborn HbE expression is dependent on the copy number of the beta E globin gene.
  • These findings offer valuable data for neonatal screening programs for Hemoglobin E.