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Identification of chromosome 21 materials using the whole chromosome 21 specific library
1Department of Pathology, School of Medical Sciences, Universiti Sains Malaysia, Kubang Kerian. Kelantan, Malaysia.
Abstract:
The chromosome in situ suppression hybridization or chromosome painting technic was applied to confirm and eliminate the markers involving chromosome 21 segments using a chromosome 21 DNA library. The library ATCCLL21SNO2 was amplified, directly biotinylated using the polymerase chain reaction. The results demonstrated a translocation of chromosome 21 material on chromosome 2 and X and eliminate the origin of the marker. Thus, the technique provides an important tool to complement the conventional G-banding technic.