Related Experiment Videos
Acetylcholine activates latent pseudorabies virus in pigs
S Tanaka1, T Imamura, M Sakaguchi
1The Chemo-Sero Therapeutic Research Institute, Kikuchi Research Center, Kumamoto, Japan.
Archives of Virology
|January 1, 1996
Summary
Acetylcholine (ACH) treatment reactivated latent Pseudorabies virus (PrV) in pigs. The virus was successfully isolated from nasal swabs and trigeminal ganglia, confirming ACH
Area of Science:
- Veterinary Virology
- Molecular Biology
- Neurovirology
Background:
- Pseudorabies virus (PrV) establishes lifelong latent infections in swine, primarily within sensory ganglia.
- Reactivation of latent viruses can lead to disease recurrence and transmission.
Purpose of the Study:
- To investigate the potential of acetylcholine (ACH) to induce the reactivation of latent Pseudorabies virus (PrV) infections in pigs.
Main Methods:
- Pigs with established latent PrV infections were treated with acetylcholine (ACH).
- Nasal swabs and cultured trigeminal ganglia from treated pigs were analyzed for PrV presence.
Main Results:
- Pseudorabies virus (PrV) was successfully isolated from both nasal swabs and cultured trigeminal ganglia of pigs following ACH treatment.
- The detection of PrV in these tissues indicates viral reactivation from latency.
Conclusions:
- Acetylcholine (ACH) acts as a stimulus that can activate latent Pseudorabies virus (PrV) infections in swine.
- This finding has implications for understanding viral latency and developing strategies to manage PrV.