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Related Experiment Videos

Interactions between Ca2+- and cAMP-dependent stimulatory pathways in parietal cells

Z Q Li1, S Mårdh

  • 1Department of Cell Biology, Faculty of Health Sciences Linköping University, Sweden.

Biochimica Et Biophysica Acta
|April 24, 1996
PubMed
Summary

Intracellular calcium (Ca2+) is crucial for cAMP-dependent secretagogue action in rat parietal cells. This study shows Ca2+ is essential for morphological changes and acid production, highlighting cross-talk between signaling pathways.

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Area of Science:

  • Cellular Physiology
  • Gastroenterology
  • Signal Transduction

Background:

  • Parietal cells secrete gastric acid, a process regulated by various signaling pathways.
  • Cyclic adenosine monophosphate (cAMP) and intracellular calcium (Ca2+) are key second messengers involved in parietal cell stimulation.
  • Understanding the interplay between cAMP and Ca2+ pathways is vital for elucidating gastric acid secretion regulation.

Purpose of the Study:

  • To investigate the role of intracellular Ca2+ in the action of cAMP-dependent secretagogues.
  • To explore the cross-talk between cAMP- and Ca2+-dependent stimulatory pathways in isolated rat parietal cells.
  • To determine the necessity of Ca2+ for morphological changes and acid production.

Main Methods:

  • Isolated rat parietal cells were utilized.

Related Experiment Videos

  • Aminopyrine accumulation was measured as an index of acid production.
  • Cytosolic free Ca2+ levels were monitored.
  • Morphological transformation and cell viability assays were performed.
  • The effects of various agents including BAPTA (Ca2+ chelator) and A23187 (Ca2+ ionophore) were assessed.

    • Main Results:

    • The increase in cytosolic free Ca2+ induced by gastrin and carbachol was blocked by BAPTA.
    • Morphological transformations induced by DBcAMP, gastrin, and Sp-cAMPS were abolished by BAPTA.
    • BAPTA dose-dependently reduced DBcAMP-stimulated aminopyrine accumulation.
    • The Ca2+ ionophore A23187 potentiated DBcAMP action and reversed BAPTA's inhibitory effects.
    • Histamine-stimulated protein kinase activity and cell viability were unaffected by BAPTA.

    Conclusions:

    • The action of cAMP-dependent secretagogues on aminopyrine accumulation and morphological transformation in parietal cells requires intracellular Ca2+.
    • Ca2+-induced morphological transformation is essential for aminopyrine accumulation.
    • A threshold level of one second messenger is necessary for the other to stimulate aminopyrine accumulation, indicating significant cross-talk.