Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

CD4 Expression by erythroid precursor cells in human bone marrow

R P Cleveland1, Y C Liu

  • 1Department of Pathology, Case Western Reserve University School of Medicine, Cleveland, OH 44109 USA.

Blood
|March 15, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Association between a novel regulatory genetic variants and lung cancer risk in Chinese: a two-stage case-control study].

Zhonghua liu xing bing xue za zhi = Zhonghua liuxingbingxue zazhi·2021
Same author

[Clinical research progress on disappearing colorectal liver metastases].

Zhonghua wei chang wai ke za zhi = Chinese journal of gastrointestinal surgery·2021
Same author

Optical design and performance of the biological small-angle X-ray scattering beamline at the Taiwan Photon Source.

Journal of synchrotron radiation·2021
Same author

[A cohort study on plasma uric acid levels and the risk of type 2 diabetes mellitus among the oldest old in longevity areas of China].

Zhonghua yi xue za zhi·2021
Same author

[The distribution of blood eosinophils and the related clinical characteristics in chronic obCOPD patients].

Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases·2021
Same author

Association of SREBF-2 gene polymorphisms with the risk of developing type 2 diabetes mellitus.

Journal of biological regulators and homeostatic agents·2021
Same journal

Fibrocytes drive JAK2V617F-mutated myelofibrosis: pitavastatin reverses marrow fibrosis and anemia.

Blood·2026
Same journal

Identifying steroid-refractory aGVHD before it happens.

Blood·2026
Same journal

ELISA-negative HIT: antibody recognition and relevance.

Blood·2026
Same journal

EBV and immunodeficiency: the odd couple drawn to the brain.

Blood·2026
Same journal

A bone to pick with ferric carboxymaltose.

Blood·2026
Same journal

A step toward streamlining HIT diagnosis.

Blood·2026
See all related articles

Researchers found a second CD4+ cell population in bone marrow, distinct from T helper cells. This population expresses erythroid markers, suggesting CD4 is present on erythroid lineage cells, impacting red blood cell production.

Area of Science:

  • Immunology
  • Hematology
  • Cell Biology

Background:

  • CD4 is a surface glycoprotein primarily expressed on T helper cells.
  • Its role in non-T cell populations, particularly in bone marrow, is less understood.
  • Human Immunodeficiency Virus type 1 (HIV-1) infects T helper cells, impacting the immune system.

Purpose of the Study:

  • To investigate CD4 expression in bone marrow specimens.
  • To identify and characterize distinct CD4-expressing cell populations.
  • To explore the potential implications of CD4 expression on erythroid lineage cells.

Main Methods:

  • Flow cytometry was utilized on 62 bone marrow specimens.
  • A lysed-whole-blood technique with monoclonal antibodies was employed for cell labeling.

Related Experiment Videos

  • Two-color flow cytometry was used to assess coexpression of lineage markers.

    • Main Results:

    • Two CD4+ cell populations were identified in 94% of specimens.
    • A novel population with low-density CD4 expression was found, present in unremarkable marrows and HIV-1 infected patients.
    • This low-density CD4 population coexpressed erythroid marker glycophorin A and myeloid markers CD13/CD33, but not CD34.

    Conclusions:

    • Evidence suggests CD4 is expressed on erythroid lineage cells in human bone marrow.
    • This finding offers a potential mechanism for direct erythroid precursor cell infection by HIV-1.
    • Expression of CD4 on erythroid cells may contribute to deranged erythropoiesis in HIV-1 infection.