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Cl/HCO(-3) exchanger is operative in isolated enterocytes from rat jejunum
M Tosco1, M N Orsenigo, A Faelli
1Dipartimento di Fisiologia e Biochimica Generali, Università di Milano, Italy.
Experientia
|March 15, 1996
Abstract:
Enterocytes isolated from rat jejunum were tested for the existence of a Cl-/HCO(-3) exchange, previously evidenced in basolateral membrane vesicles but not in brush border. Cells were found to retain functional integrity and transport capabilities long enough to allow Cl- fluxes to be measured. Both efflux and uptake experiments indicate that a Cl-/HCO(-3) antiport, inhibited by 4,4'-diisothiocyanostilbene-2-2'-disulfonic acid (DIDS), is functional under resting conditions.