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Tissue factor expression in human leukemic cells

G A Hair1, S Padula, R Zeff

  • 1The University of Connecticut School of Medicine, Farmington, USA.

Leukemia Research
|January 1, 1996
PubMed
Summary
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Tissue factor (TF) procoagulant activity (PCA) in leukemia cells is complex. Studies show TF expression varies with cell differentiation, suggesting novel regulatory mechanisms in acute leukemia patients.

Area of Science:

  • Hematology
  • Molecular Biology
  • Oncology

Background:

  • Acute leukemia patients face thrombotic and hemorrhagic risks, especially during chemotherapy.
  • Tissue factor (TF) procoagulant activity (PCA) release is implicated in these complications.
  • TF expression in normal monocytes contrasts with the unclear origin in leukemic cells.

Purpose of the Study:

  • To investigate TF expression and regulation in leukemic cell lines and primary cells.
  • To characterize TF mRNA, antigen (TF:Ag), and PCA in HL-60 cells and primary leukocytes.
  • To explore potential novel regulatory mechanisms of TF PCA in leukemia.

Main Methods:

  • Utilized full-length cDNA probe, monoclonal antibodies (MAbs), and a one-stage PCA assay.
  • Studied HL-60 cells, human monocytes/macrophages (Mo/Mø), and polymorphonuclear leukocytes (PMN).

Related Experiment Videos

  • Analyzed TF expression following induced differentiation (macrophage and granulocyte lineages).

    • Main Results:

    • Detected TF mRNA and TF:Ag in HL-60 cells; PCA increased with macrophage differentiation.
    • Granulocyte differentiation of HL-60 cells decreased TF mRNA without altering TF:Ag.
    • Pure PMN showed no TF PCA, TF:Ag, or TF mRNA; mature Mo/Mø showed persistent TF:Ag.

    Conclusions:

    • Discordant TF expression in HL-60 cells suggests novel post-synthetic regulatory mechanisms.
    • Cellular differentiation level may influence TF PCA expression in leukemia.
    • These mechanisms could explain TF gene product activation of coagulation in mixed-lineage leukemia cells.