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Identification of a HeLa mRNA fraction which corrects the mitomycin C sensitivity of irs1 cells
O Bender1, N J Jones, K Sperling
1Institut für Humangenetik, Virchow Klinikum, Humboldt Universität zu Berlin, Germany.
Mutation Research
|May 15, 1996
Abstract:
The hamster cell mutant irs1 is defective in its response to DNA lesions caused by a variety of mutagens, particularly cross-linking agents. These cells have been assigned to complementation group 2 of X-ray-sensitive mutants and the mutated gene is called XRCC2(X-ray repair cross complementing). We have identified, by microinjection, a human mRNA fraction which can transiently correct the sensitivity of these cells to cross-linking agents. This fraction contains mRNAs of 3.5 kb (+/- 0.25) including, therefore, the transcript of the XRCC2 gene.