Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Spermatogenesis01:41

Spermatogenesis

91.1K
Spermatogenesis is the process by which haploid sperm cells are produced in the male testes. It starts with stem cells located close to the outer rim of seminiferous tubules. These spermatogonial stem cells divide asymmetrically to give rise to additional stem cells (meaning that these structures “self-renew”), as well as sperm progenitors, called spermatocytes. Importantly, this method of asymmetric mitotic division maintains a population of spermatogonial stem cells in the male...
91.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Comparative analysis of SV40 17kT and LT function in vivo demonstrates that LT's C-terminus re-programs hepatic gene expression and is necessary for tumorigenesis in the liver.

Oncogenesis·2013
Same author

Generation of transgenic mice from yeast artificial chromosome DNA that has been modified by gene targeting.

Trends in cardiovascular medicine·2011
Same author

PP2A-dependent disruption of centrosome replication and cytoskeleton organization in Drosophila by SV40 small tumor antigen.

Oncogene·2008
Same author

The transcriptional regulatory strategy of the rat tissue kallikrein gene family.

Genes and function·2007
Same author

Disruption of PPT1 or PPT2 causes neuronal ceroid lipofuscinosis in knockout mice.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Decreased lipid synthesis in livers of mice with disrupted Site-1 protease gene.

Proceedings of the National Academy of Sciences of the United States of America·2001

Related Experiment Video

Updated: May 6, 2026

Step-specific Sorting of Mouse Spermatids by Flow Cytometry
06:31

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

10.6K

Rat spermatogenesis in mouse testis

D E Clouthier1, M R Avarbock, S D Maika

  • 1Laboratory of Reproductive Physiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia 19104, USA.

Nature
|May 30, 1996
PubMed
Summary
This summary is machine-generated.

Spermatogonial stem cell transplantation from rats to mice successfully generated rat sperm. This xenogeneic approach shows promise for interspecies germline stem cell applications and fertility restoration.

More Related Videos

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
10:22

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Published on: September 17, 2019

13.0K
Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

1.7K

Related Experiment Videos

Last Updated: May 6, 2026

Step-specific Sorting of Mouse Spermatids by Flow Cytometry
06:31

Step-specific Sorting of Mouse Spermatids by Flow Cytometry

Published on: December 31, 2015

10.6K
Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment
10:22

Enrichment of Pachytene Spermatocytes and Spermatids from Mouse Testes Using Standard Laboratory Equipment

Published on: September 17, 2019

13.0K
Mouse Round Spermatid Injection
08:41

Mouse Round Spermatid Injection

Published on: January 26, 2024

1.7K

Area of Science:

  • Reproductive Biology
  • Stem Cell Biology
  • Xenotransplantation

Background:

  • Spermatogonial stem cells (SSCs) are crucial for male fertility.
  • Previous studies demonstrated successful SSC transplantation within mice.
  • The potential for interspecies SSC transplantation remained largely unexplored.

Purpose of the Study:

  • To investigate the feasibility of xenogeneic SSC transplantation.
  • To determine if rat SSCs can establish spermatogenesis in a mouse testicular environment.
  • To assess the potential for generating functional spermatozoa from transplanted cells of another species.

Main Methods:

  • Transplantation of marked rat testis cells into immunodeficient mouse testes.
  • Monitoring the development of spermatogenesis in recipient mice over time.
  • Morphological analysis of spermatozoa in recipient epididymides.

Main Results:

  • Rat spermatogenesis was successfully established in 19 out of 20 mouse testes from 10 recipients.
  • Rat spermatozoa with normal morphology were found in the epididymides of mice with transplants exceeding 110 days.
  • The study demonstrated successful xenogeneic engraftment and differentiation of SSCs.

Conclusions:

  • Spermatogonial stem cells from one species can generate functional gametes in the testes of another species.
  • This research validates the potential of SSC transplantation for xenogeneic spermatogenesis.
  • The findings open avenues for interspecies germline stem cell applications and potential fertility treatments.