Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Probe-immobilized affinity chromatography/mass spectrometry

A H Brockman1, R Orlando

  • 1Complex Carbohydrate Research Center, University of Georgia, Athens 30602-4712, USA.

Analytical Chemistry
|December 15, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Factors affecting seroreversion by 6 months among healthcare workers in Connecticut.

The Journal of hospital infection·2021
Same author

The bacterial quorum sensing molecule, 2-heptyl-3-hydroxy-4-quinolone (PQS), inhibits signal transduction mechanisms in brain tissue and is behaviorally active in mice.

Pharmacological research·2021
Same author

Seroprevalence of SARS-CoV-2 antibodies, associated epidemiological factors and antibody kinetics among healthcare workers in Connecticut.

The Journal of hospital infection·2021
Same author

Letter: the addition of an immunosuppressant in patients with unsatisfactory response to vedolizumab.

Alimentary pharmacology & therapeutics·2018
Same author

Large-Scale Condensed Matter DFT Simulations: Performance and Capabilities of the CRYSTAL Code.

Journal of chemical theory and computation·2017
Same author

Managing complex respiratory patients in the community: an evaluation of a pilot integrated respiratory care service.

BMJ open respiratory research·2017

A novel probe affinity mass spectrometry (MS) technique enables rapid, in situ identification of biomolecules directly on a matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) probe surface. This method combines affinity chromatography selectivity with MALDI-MS sensitivity for diverse life science applications.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Biotechnology

Background:

  • Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a sensitive technique for molecular weight determination.
  • Affinity chromatography offers high selectivity for biomolecule separation.
  • Integrating separation and detection methods can enhance analytical efficiency.

Purpose of the Study:

  • To develop a novel technique for in situ affinity separations directly on a MALDI-MS probe.
  • To combine the selectivity of immobilized affinity chromatography with the sensitivity of MALDI-MS.
  • To enable rapid identification of biomolecules and other compounds from complex mixtures.

Main Methods:

  • Development of a probe affinity MS technique.
  • Immobilization of affinity ligands onto the MALDI-MS probe surface.

Related Experiment Videos

  • Direct analysis of complex biological mixtures using the integrated system.

    • Main Results:

    • Successful in situ affinity separations were achieved directly on the MALDI-MS probe.
    • The technique provided simultaneous biomolecule identification and molecular weight information.
    • Rapid and facile analysis of antigens and ligands from complex biological samples was demonstrated.

    Conclusions:

    • Probe affinity MS is a powerful new technique for analyzing complex biological mixtures.
    • This method offers a rapid and sensitive approach for identifying biomolecules and other compounds.
    • The technique has broad applicability across various life science disciplines.