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Identification of multiple plasmids released from recombinant genomes of Hansenula polymorpha by transformation of
1Fachbereich Biologie, Universität Oldenburg, Germany.
Applied and Environmental Microbiology
|May 1, 1996
Abstract:
Total DNAs isolated from two Hansenula polymorpha (Pichia angusta) strains having chromosomal single or tandem multiple integrations of a pUC18-derived expression plasmid produced Escherichia coli transformants which contained plasmids of different size and/or organization than that of the expression plasmid. Evidence that plasmid-like structures are formed in H. polymorpha and that their formation is stimulated by DNA damage is presented in this study.