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An on-line semi-automated solid-phase extraction procedure for high-performance liquid chromatographic determination
C Bottalico1, G Micelli, A Guerrieri
1Istituto Oncologico, Bari, Italy.
Journal of Pharmaceutical and Biomedical Analysis
|October 1, 1995
Abstract:
A semi-automated solid-phase extraction procedure on-line with gradient elution reversed-phase chromatography permits the determination of lonidamine and its metabolite in human serum. The average recovery from serum at the 2.5 micrograms ml-1 level was (92.8 +/- 3.4)%. The limit of quantitation for a 100 microliter sample size was 50 ng ml-1. The within-day (n = 5) and between-day (n = 5) relative standard deviations for lonidamine determination in serum samples spiked at the 2.5 micrograms ml-1 level were 2.7% and 4.5%, respectively.