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Related Experiment Videos

Antigen-induced anaphylactic death in mice

H Y Lei1, S H Lee, S H Leir

  • 1Department of Microbiology and Immunology, National Cheng Kung University College of Medicine, Tainan, Taiwan, Republic of China.

International Archives of Allergy and Immunology
|April 1, 1996
PubMed
Summary

This study reveals a non-immunoglobulin E (IgE)-mediated anaphylactic death in mice, triggered by bovine serum albumin (BSA). This reaction involves histamine and serotonin, leading to fatal blood volume loss and vasopermeability.

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Area of Science:

  • Immunology
  • Allergy Research
  • Pharmacology

Background:

  • Anaphylaxis is a severe allergic reaction often mediated by immunoglobulin E (IgE).
  • The mechanisms underlying non-IgE-mediated anaphylaxis require further investigation.
  • Bovine serum albumin (BSA) is a common antigen used in immunological studies.

Purpose of the Study:

  • To investigate the induction and characteristics of anaphylactic death in mice using BSA.
  • To identify the immunological mediators involved in BSA-induced anaphylaxis.
  • To explore potential therapeutic interventions for this condition.

Main Methods:

  • Intravenous sensitization of ICR mice with BSA/CFA.
  • Dose-response and route-of-administration studies for BSA challenge.

Related Experiment Videos

  • Evaluation of different adjuvants and antigens for sensitization.
  • Assessment of mouse strain and gender susceptibility.
  • Pharmacological blockade of histamine and serotonin receptors.
  • Passive transfer of anaphylaxis using immune serum.
  • Analysis of IgG subclasses and IgE levels.

    • Main Results:

    • Intravenous BSA injection induced rapid anaphylactic death in sensitized mice, persisting for over 3 months.
    • The response was dose-dependent, route-specific (IV > IP > SC), and influenced by mouse strain and gender.
    • Antigens like HoGG induced higher mortality than GAT or lysozyme.
    • Cyproheptadine, diphenhydramine, and ketanserin prevented death, as did saline infusion.
    • Immune serum could transfer the reaction, and the activity was heat-stable.
    • Primarily IgG1 was detected, with no detectable IgE.

    Conclusions:

    • BSA-induced anaphylactic death in mice is mediated by a non-IgE pathway.
    • Histamine and serotonin release are key mediators, causing increased vascular permeability and blood loss.
    • This model offers insights into non-IgE-mediated hypersensitivity reactions.