Related Experiment Videos
Radioassay of soluble immune complexes using their uptake by macrophage Fc receptors
Sera from patients were tested for the presence of soluble immune complexes (IC) by a method based on the observation that IC inhibit uptake of aggregated IgG by the Fc receptors of macrophages. From the saturation curve of macrophages with 125I-labelled aggregates the optimal inhibitory effect of a measured amount of IC was determined. This radioassay is highly sensitive, being capable of detecting 20 ng of IC, is reproducible and covers a wide range of measurement (20-2000 ng).
Sera from patients were tested for the presence of soluble immune complexes (IC) by a method based on the observation that IC inhibit uptake of aggregated IgG by the Fc receptors of macrophages. From the saturation curve of macrophages with 125I-labelled aggregates the optimal inhibitory effect of a measured amount of IC was determined. This radioassay is highly sensitive, being capable of detecting 20 ng of IC, is reproducible and covers a wide range of measurement (20-2000 ng).