Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Rapid, specific and sensitive method for isoniazid determination in serum

N Sadeg1, N Pertat, H Dutertre

  • 1Laboratoire Claude Bernard, Hôpital René Dubos, Pontoise, France.

Journal of Chromatography. B, Biomedical Applications
|January 12, 1996
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Determination serique de la crimidine par clhp/es/sm chez un patient ayant ingere un « souricide foudroyant ».

Acta clinica Belgica·2014
Same author

[Introducing the new international vocabulary of metrology].

Annales de biologie clinique·2009
Same author

[Recommendations for metrological control of measuring equipments in the clinical laboratories].

Annales de biologie clinique·2009
Same author

[Recommendations for expressing uncertainty of measurement of quantitative results in laboratory medicine].

Annales de biologie clinique·2007
Same author

[Monitoring of adipocyte responsiveness by in situ microdialysis in lipodystrophy tissue: adjustment of a glycerol quantification method in small samples].

Acta clinica Belgica·2006
Same author

[Prescription, assay and interpretation of cardiac troponins tests: guidelines from SFBC-CNBC troponin working group].

Annales de biologie clinique·2005

A new high-performance liquid chromatography method accurately measures isoniazid (INH) in human serum. This simple assay offers reliable drug determination for clinical applications.

Area of Science:

  • Analytical Chemistry
  • Pharmacokinetics
  • Biomedical Analysis

Background:

  • Accurate quantification of isoniazid (INH) in human serum is crucial for therapeutic drug monitoring.
  • Existing methods may lack simplicity, specificity, or speed for routine clinical use.

Purpose of the Study:

  • To develop and validate a simple, specific, and rapid high-performance liquid chromatographic (HPLC) assay for determining INH concentrations in human serum.

Main Methods:

  • Serum samples were treated with trichloroacetic acid for protein precipitation.
  • Isoniazid was derivatized with trans-cinnamaldehyde, forming a UV-absorbing compound at 340 nm.
  • HPLC analysis utilized a reversed-phase C18 column with a specific mobile phase composition.

Main Results:

Related Experiment Videos

  • The assay demonstrated excellent extraction recovery (averaging 100%) for INH in human serum.
  • High precision was observed, with within-day coefficients of variation ranging from 1.2% to 3.5% and day-to-day variations from 2.8% to 4.3%.

Conclusions:

  • The developed HPLC method provides a simple, specific, rapid, and reliable means for quantifying isoniazid in human serum.
  • This assay is suitable for routine clinical laboratory analysis and pharmacokinetic studies.