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Rapid high-performance liquid chromatographic assay for atovaquone
A G Hansson1, S Mitchell, P Jatlow
1Department of Laboratory Medicine, Yale University School of Medicine, New Haven, CT 06520-8035, USA.
Journal of Chromatography. B, Biomedical Applications
|January 12, 1996
Summary
A new high-performance liquid chromatography assay accurately measures atovaquone levels in plasma. This method is crucial for monitoring treatment of opportunistic infections in individuals with acquired immunodeficiency syndrome (AIDS).
Area of Science:
- Analytical Chemistry
- Pharmacology
Background:
- Atovaquone is a key drug for treating Pneumocystis carinii pneumonia and Toxoplasma gondii encephalitis.
- These infections are common complications in patients with acquired immunodeficiency syndrome (AIDS).
- Accurate therapeutic drug monitoring is essential for effective treatment of these opportunistic infections.
Purpose of the Study:
- To develop and validate a rapid, high-performance liquid chromatography (HPLC) assay for quantifying atovaquone in human plasma.
- To establish a reliable method for therapeutic drug monitoring of atovaquone in AIDS patients.
Main Methods:
- Plasma samples were deproteinized using acetonitrile-aqueous 1% acetic acid (85:15).
- Atovaquone was separated on a C6 column using a mobile phase of methanol-10 mM triethylamine in aqueous 0.2% trifluoroacetic acid (76:24).
- Detection was performed at 254 nm.
Main Results:
- The assay demonstrated a working range of 0.5 to 50 micrograms/ml.
- Excellent recovery (97%) was achieved.
- Good precision was observed with between-day coefficients of variation of 2.1% at 50 micrograms/ml and 10.3% at 1 microgram/ml.
- Commonly used AIDS medications did not interfere with the assay.
Conclusions:
- A rapid and reliable HPLC assay for atovaquone in plasma has been successfully developed.
- This assay is suitable for therapeutic drug monitoring in patients with AIDS-related opportunistic infections.
- The method offers good sensitivity, recovery, and precision, with minimal interference from other drugs.