Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hepatitis E virus (87A strain) propagated in A549 cells

R Huang1, N Nakazono, K Ishii

  • 1Department of Public Health, Kansai Medical University, Osaka, Japan.

Journal of Medical Virology
|December 1, 1995
PubMed
Summary

The 87A strain of hepatitis E virus (HEV) successfully replicated in A549 cells, demonstrating significant cytopathic effects. This study confirms A549 cells as a viable host for HEV propagation and characterization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Genetic deletion of the Pten tumor suppressor gene promotes cell motility by activation of Rac1 and Cdc42 GTPases.

Current biology : CB·2001
Same author

Changing sexual attitudes and behaviour in China: implications for the spread of HIV and other sexually transmitted diseases.

AIDS care·2001
Same author

Pharmacokinetics of 2-hydroxyflutamide, a major metabolite of flutamide, in normal and CCl4-poisoned rats.

Zhongguo yao li xue bao = Acta pharmacologica Sinica·2000
Same author

Flutamide suppressed prostate hypertrophy in rats and mice.

Zhongguo yao li xue bao = Acta pharmacologica Sinica·2000
Same author

Type XV collagen in human colonic adenocarcinomas has a different distribution than other basement membrane zone proteins.

Human pathology·2000
Same author

Carvedilol prevents epinephrine-induced apoptosis in human coronary artery endothelial cells: modulation of Fas/Fas ligand and caspase-3 pathway.

Cardiovascular research·2000

Area of Science:

  • Virology
  • Cell Biology
  • Hepatitis Research

Background:

  • Hepatitis E virus (HEV) 87A strain was previously isolated in 2BS cells.
  • HEV is a significant cause of viral hepatitis globally.

Purpose of the Study:

  • To propagate the HEV 87A strain in A549 cells.
  • To characterize HEV replication and effects in this new cell line.

Main Methods:

  • Propagation of HEV 87A strain in A549 cell monolayers.
  • Observation of cytopathic effects (CPE).
  • Detection of HEV-RNA using polymerase chain reaction (PCR) with specific primers.
  • Sequencing of PCR products.

Main Results:

  • A marked cytopathic effect was observed in A549 cells infected with HEV 87A.

Related Experiment Videos

  • HEV-RNA was detected in cell supernatants across multiple passages.
  • PCR analysis confirmed the presence of HEV with a 239 bp band.
  • Sequencing of the fourth passage confirmed viral presence and replication.

    • Conclusions:

    • A549 cells support the replication of the HEV 87A strain.
    • The study demonstrates the utility of A549 cells for HEV propagation and study.
    • The observed cytopathic effect indicates potential for further research into HEV pathogenesis.