Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antisense globin RNA in mouse erythroid tissues: structure, origin, and possible function

V Volloch1, B Schweitzer, S Rits

  • 1Boston Biomedical Research Institute, Massachusetts 02114, USA.

Proceedings of the National Academy of Sciences of the United States of America
|March 19, 1996
PubMed
Summary

Researchers identified antisense beta-globin RNA in mouse erythroid cells. This antisense RNA is complementary to mRNA and may play a role in generating new sense-strand globin mRNA.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dyspnoea: Pathophysiology and a clinical approach.

South African medical journal = Suid-Afrikaanse tydskrif vir geneeskunde·2016
Same author

Postoperative pain assessment in children: a pilot study of the usefulness of the analgesia nociception index.

British journal of anaesthesia·2015
Same author

Effects of β2 agonists on post-thoracotomy pain incidence.

European journal of pain (London, England)·2015
Same author

A socio-ecological approach promoting physical activity and limiting sedentary behavior in adolescence showed weight benefits maintained 2.5 years after intervention cessation.

International journal of obesity (2005)·2014
Same author

Growth limitation of planktonic bacteria in a large mesotrophic lake.

Microbial ecology·2013
Same author

Use of dissolved carbohydrates by planktonic bacteria in a mesotrophic lake.

Microbial ecology·2013

Area of Science:

  • Molecular Biology
  • Genetics
  • RNA Biology

Background:

  • Antisense RNA molecules are known to regulate gene expression.
  • The presence and function of antisense globin RNA in erythroid development were previously unclear.

Purpose of the Study:

  • To detect and characterize antisense beta-globin RNA in mouse erythroid tissues.
  • To investigate the structural features and potential function of this antisense RNA.

Main Methods:

  • Utilized a multistep RNA ligation procedure with a molecular tag.
  • Preserved RNA termini to identify sense vs. antisense molecules.
  • Analyzed antisense RNA complementarity to spliced globin mRNA.

Main Results:

Related Experiment Videos

  • Identified and characterized antisense beta-globin RNA in erythroid spleen cells and reticulocytes.
  • Antisense RNA is fully complementary to spliced globin mRNA, suggesting a template/transcript relationship.
  • Characterized three forms of antisense RNA based on 3' termini: full-size, truncated, and extended.

    • Conclusions:

    • Antisense beta-globin RNA exists in mouse erythroid cells and is complementary to mRNA.
    • Structural features, including a uridylate stretch and complementary sequences, suggest a role in globin mRNA regulation.
    • The findings propose a mechanism by which antisense RNA may generate new sense-strand globin mRNA.