Related Experiment Videos
GS28, a 28-kilodalton Golgi SNARE that participates in ER-Golgi transport
V N Subramaniam1, F Peter, R Philp
1Membrane Biology Laboratory, Institute of Molecular and Cell Biology, National University of Singapore.
Science (New York, N.Y.)
|May 24, 1996
Abstract:
Little is known about the integral membrane proteins that participate in the early secretory pathway of mammalian cells. The complementary DNA encoding a 28-kilodalton protein (p28) of the cis-Golgi was cloned and sequenced. The protein was predicted to contain a central coiled-coil domain with a carboxyl-terminal membrane anchor. An in vitro assay for endoplasmic reticulum-Golgi transport was used to show that p28 participates in the docking and fusion stage of this transport event. Biochemical studies established that p28 is a core component of the Golgi SNAP receptor (SNARE) complex.