Related Experiment Videos
Evidence that two genomic species of Rhizobium are associated with Medicago truncatula
1Laboratoire d'Ecologie Microbienne du Sol, URA-CNRS 1977, Université Lyon I, Villeurbanne, France.
Archives of Microbiology
|April 1, 1996
Abstract:
Seventy-three isolates of rhizobia sampled from root nodules of Medicago truncatula were analyzed by restriction fragment length polymorphism (RFLP) of DNA regions amplified by the polymerase chain reaction (PCR) targeting the symbiotic plasmid (nifD-K, nodD1, and nodD2 genes) and the chromosome (16S rDNA plus intergenic spacer). Two genotypic groups were found, regardless of the DNA region targeted. These two groups were given the status of genomic species based on results of DNA/DNA hybridization.