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P2X1 receptor activation in HL60 cells
1Glaxo Institute for Molecular Biology, Geneva, Switzerland.
Blood
|April 1, 1996
Summary
Differentiated HL60 cells express functional P2X1 receptors. These receptors are chronically desensitized by endogenously released adenosine triphosphate (ATP), requiring apyrase or suramin treatment for detection.
Area of Science:
- Cell Biology
- Neuroscience
- Pharmacology
Background:
- The human P2X1 receptor, a ligand-gated ion channel, is crucial in various physiological processes.
- High messenger RNA levels for P2X1 receptors were recently identified in differentiated promyelocytes (HL60 cells).
Purpose of the Study:
- To confirm the expression and functionality of P2X1 receptors in differentiated HL60 cells.
- To investigate the influence of endogenous adenosine triphosphate (ATP) on P2X1 receptor activity in these cells.
Main Methods:
- Radioligand binding assays and immunohistochemistry were used to detect P2X1 receptor protein.
- Electrophysiology was employed to measure receptor currents.
- The luciferin-luciferase assay was utilized for direct measurement of ATP release.
Main Results:
- P2X1 receptor protein expression was confirmed in HL60 cells.
- Adenosine triphosphate (ATP)-elicited currents exhibited P2X1 receptor characteristics but required pretreatment with apyrase or suramin.
- HL60 cells were found to release ATP, leading to chronic receptor desensitization.
Conclusions:
- Functional P2X1 receptors are present in the plasma membrane of differentiated HL60 cells.
- Endogenous ATP release from HL60 cells desensitizes P2X1 receptors, impacting their measurable activity.