The determination of corticoids and related steroid analogs by high-performance liquid chromatography

High-performance reverse-phase liquid chromatographic data on 28 corticoids and related steroid analogs, plus methyl and propyl parabens, are presented. Retention values, relative to acetone, are reported for each steroid on Bondapak Phenyl/Corasil, Bondapak C18/Corasil, and micro(micron) Bondapak C18 columns, and methanol/water or acetonitrile/water mobile phases. Ultraviolet detector response data for four of the steroids and calculated efficiencies for the three different reverse-phase columns are given. Chromatograms showing the separations of four steroids plus methyl and propyl parabens obtained on each of the three columns are also presented.