Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

The chromosome complement of human uncleaved oocytes

R M Kumar1, A Khuranna

  • 1Department of Obstetrics and Gynaecology, Faculty of Medicine and Health Sciences, U.A.E. University, Al Ain, UAE.

Journal of Obstetrics and Gynaecology (Tokyo, Japan)
|December 1, 1995
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of surgery report cards on improving radical prostatectomy quality: the SuRep study protocol.

BMC urology·2018
Same author

Cutaneous metastasis of renal cell carcinoma masquerading as an infected sebaceous cyst.

Journal of stomatology, oral and maxillofacial surgery·2017
Same author

Metastatic renal cell carcinoma without evidence of a primary renal tumour.

Current oncology (Toronto, Ont.)·2014
Same author

Constipation in children--is it always benign?

Colorectal disease : the official journal of the Association of Coloproctology of Great Britain and Ireland·2011
Same author

A comparison of the clinical profile of Kawasaki disease in children from Northern India above and below 5 years of age.

Clinical and experimental rheumatology·2007
Same author

Azygos continuation of interrupted inferior vena cava in association with sick sinus syndrome.

Heart (British Cardiac Society)·2005

Chromosomal abnormalities in human oocytes are frequent, affecting nearly half of those that fail to fertilize. This high rate of chromosomal disorders may explain in vitro fertilization (IVF) failure, especially in older women.

Area of Science:

  • Reproductive biology
  • Human genetics
  • Cytogenetics

Background:

  • Investigating human oocytes that fail to fertilize is crucial for understanding reproductive failure.
  • Cytogenetic analysis of unfertilized oocytes can reveal underlying chromosomal abnormalities.

Purpose of the Study:

  • To determine the type and frequency of chromosomal abnormalities in human oocytes that failed to fertilize.
  • To assess the contribution of chromosomal aberrations to in vitro fertilization (IVF) failure.

Main Methods:

  • Oocyte recovery from patients undergoing IVF procedures.
  • In vitro preincubation and insemination of oocytes.
  • Karyotyping of 121 uncleaved oocytes 50 hours post-insemination.

Main Results:

Related Experiment Videos

  • 47.6% of successfully karyotyped oocytes exhibited chromosomal aberrations.
  • Aneuploidy was the most common abnormality (34.8%), significantly higher in women over 35.
  • Other abnormalities included diploidy (7.2%), hyperploidy (2.8%), and translocations (2.8%).
  • Premature sperm chromosome condensation occurred in 18.8% of oocytes.

Conclusions:

  • The high prevalence of chromosomal disorders in unfertilized human oocytes likely contributes to IVF failure.
  • Maternal age is a significant factor associated with increased aneuploidy rates.