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Characterization of an N6-oxopropenyl-2'-deoxyadenosine adduct in malondialdehyde-modified DNA using liquid
A K Chaudhary1, G R Reddy, I A Blair
1A.B. Hancock Jr Memorial Laboratory for Cancer Research, Vanderbilt University School of Medicine, Nashville, TN 37232, USA.
Abstract:
Malondialdehyde (MDA), a product of lipid peroxidation, causes mutations in bacterial and mammalian cells and cancer in rats. MDA reacts with deoxynucleosides in vitro and the monomeric adduct of MDA with deoxyguanosine (M1G-dR) is the major adduct. M1G-dR has been detected in rat and human liver. Random mutagenesis studies with MDA-modified DNA and recent 32P-postlabeling studies indicate that in addition to M1G-dR, adducts to deoxyadenosine may also be formed. We have utilized liquid chromatography coupled with electrospray ionization tandem mass spectrometry to characterize an N6-oxopropenyl-2'-deoxyadenosine adduct (M1A-dR) in calf DNA modified with MDA.