Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bone sialoprotein stimulates in vitro bone resorption

C Raynal1, P D Delmas, C Chenu

  • 1INSERM U-403, Hôpital Edouard Herriot, Lyon, France.

Endocrinology
|June 1, 1996
PubMed
Summary

Bone sialoprotein (BSP) significantly stimulates bone resorption and osteoclast formation in vitro. This protein, crucial for bone, may regulate bone remodeling through its RGD sequence and other molecular regions.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Pain after osteoporotic fractures using mouse models and patient samples.

Journal of bone and mineral metabolism·2025
Same author

ECFS standards of care on CFTR-related disorders: Identification and care of the disorders.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2024
Same author

The multi-faceted nature of 15 CFTR exonic variations: Impact on their functional classification and perspectives for therapy.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2022
Same author

Fracture-induced pain-like behaviours in a femoral fracture mouse model.

Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·2021
Same author

Penetrance is a critical parameter for assessing the disease liability of CFTR variants.

Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society·2020
Same author

Variant classifications, databases and genotype-phenotype correlations.

Archives de pediatrie : organe officiel de la Societe francaise de pediatrie·2020

Area of Science:

  • Biochemistry
  • Cell Biology
  • Bone Biology

Background:

  • Bone sialoprotein (BSP) is a bone-specific protein containing an arginine-glycine-aspartic acid (RGD) sequence.
  • The RGD sequence mediates cell adhesion via the vitronectin receptor, implicating BSP in osteoclast activity.

Purpose of the Study:

  • To investigate the role of BSP in in vitro bone resorption.
  • To elucidate the mechanisms by which BSP influences osteoclast behavior and bone remodeling.

Main Methods:

  • Isolated osteoclast resorption pit assay using ivory slices.
  • Dose-response experiments with varying concentrations of BSP.
  • Coculture system of bone marrow and osteoblastic cells to assess osteoclast formation.

Main Results:

  • BSP significantly stimulated bone resorption in a dose-dependent manner, increasing resorbed area and lacunae formation.
  • BSP enhanced osteoclast adhesion to bone matrix, likely via its RGD sequence.
  • While stimulating resorption, BSP inhibited osteoclast-like cell formation in coculture models.

Conclusions:

  • BSP plays a critical role in the bone resorption process.
  • BSP may regulate both bone resorption and osteoclastogenesis, potentially through its RGD and acidic sequences.
  • BSP's dual role suggests complex involvement in bone remodeling and homeostasis.

Related Experiment Videos