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EagI and NotI linking clones from human chromosomes 11 and Xp
M A Pook1, R Thakrar, B Pottinger
1MRC Molecular Endocrinology Group, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Human Genetics
|June 1, 1996
Summary
Researchers created DNA libraries from human chromosomes 11 and Xp, identifying 66 clones. These clones aid in detailed mapping and identifying genes linked to CpG-rich islands.
Area of Science:
- Genomics
- Human Genetics
- Molecular Biology
Background:
- Mouse-human somatic cell hybrids are valuable tools for gene mapping.
- Previous mapping efforts have established a foundation for further genomic analysis.
Purpose of the Study:
- To construct and regionally localize DNA clones from human chromosomes 11 and Xp.
- To facilitate the generation of detailed physical maps and identify novel genes.
Main Methods:
- Preparation of EagI and NotI linking libraries from a mouse-human somatic cell hybrid (1W1LA4.9).
- Isolation and characterization of human DNA clones using hybridization and restriction enzyme digestion.
- Regional mapping of clones using a panel of somatic cell hybrids with chromosome deletions.
- Assessment of microsatellite repetitive sequences and restriction fragment length polymorphisms.
Main Results:
- 66 human DNA clones (52 EagI, 14 NotI) were isolated and mapped.
- 39 clones localized to chromosome 11, with significant clusters in 11q13 and 11q14-q23.1.
- 27 clones localized to chromosome Xp, with a cluster in Xp11.
- Microsatellite analysis and RFLPs were identified for selected clones.
Conclusions:
- The generated EagI and NotI clones provide valuable resources for high-resolution mapping of human chromosomes 11 and Xp.
- These clones will aid in the identification of genes associated with CpG-rich islands and genetic disorders.
- This study contributes to the ongoing efforts in constructing comprehensive human genome maps.
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