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Establishment of a cell line from Indian bonnet monkey (Macaca radiata) kidney and its characterization
S S Gogate1, K Banerjee, S V Gangodkar
1National Institute of Virology, Pune, India.
Abstract:
A cell line (MRK-90) has been established from a kidney tissue of a macaque monkey (Macaca radiata) of India. The cells are in 150th passage and have been characterized for morphology, chromosome number, isoenzyme patterns (LDH and MDH) and virus susceptibility. These studies indicate that the cells are epithelial like, heteroploid (2n = 65) and grow easily on glass surface/plastic surface without any difficulty. The cells are susceptible to a broad spectrum of viruses.
Insights
A new macaque kidney cell line, MRK-90, was established from Indian rhesus monkeys (Macaca radiata). This epithelial-like, heteroploid cell line is easily cultured and broadly susceptible to viruses, making it a valuable research tool.
Area of Science:
- * Virology
- * Cell Biology
- * Primate Research
Background:
- * Establishment of novel cell lines is crucial for advancing virology and cell biology research.
- * Primate-derived cell lines offer unique physiological relevance for studying diseases and developing vaccines.
- * The Indian rhesus monkey (Macaca radiata) is a significant model organism in biomedical research.
Purpose of the Study:
- * To establish and characterize a new cell line from the kidney tissue of Macaca radiata.
- * To assess the cell line's suitability for virological studies through comprehensive characterization.
- * To provide a novel research platform for infectious disease research.
Main Methods:
- * Cell line establishment from Macaca radiata kidney tissue.
- * Characterization of morphology, chromosome number (karyotyping), and isoenzyme patterns (LDH, MDH).
- * Assessment of virus susceptibility across a broad spectrum of viral agents.
Main Results:
- * A new epithelial-like cell line, MRK-90, was successfully established and maintained through 150 passages.
- * The cell line was confirmed to be heteroploid with a modal chromosome number of 2n = 65.
- * MRK-90 cells demonstrated easy growth on standard culture surfaces and susceptibility to a wide range of viruses.
Conclusions:
- * The MRK-90 cell line represents a valuable new resource for biomedical research, particularly in virology.
- * Its characteristics, including ease of culture and broad virus susceptibility, make it suitable for diverse experimental applications.
- * This cell line can facilitate studies involving primate-specific viruses and contribute to vaccine development and disease modeling.