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Peroxisomes in pulmonate gastropods

E Dannen, M E Beard

    The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
    |May 1, 1977
    PubMed
    Summary

    Peroxisomes were identified in the kidney sacs of two snail species. Differences in their structure and response to the 3,3′-diaminobenzidine stain suggest biochemical variations between species.

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    Area of Science:

    • Cell biology
    • Invertebrate zoology
    • Biochemistry

    Background:

    • Peroxisomes are vital organelles involved in metabolic processes.
    • Their presence and function in terrestrial gastropods are not well-documented.
    • Kidney sacs in gastropods play a role in excretion and homeostasis.

    Purpose of the Study:

    • To investigate the presence and morphology of peroxisomes in the kidney sac cells of two terrestrial pulmonate gastropods: Arion ater and Ariolimax columbianus.
    • To compare the cytochemical characteristics of these peroxisomes, particularly their reaction to the 3,3′-diaminobenzidine (DAB) staining technique.

    Main Methods:

    • Morphological analysis of kidney sac cells using electron microscopy.
    • Application of the 3,3′-diaminobenzidine (DAB) cytochemical staining technique to identify peroxisomal enzymes.
    • Acid-phosphatase staining to differentiate peroxisomes from lysosomes.

    Main Results:

    • Organelles consistent with peroxisomes were observed in both Arion ater and Ariolimax columbianus kidney sac cells.
    • Arion ater peroxisomes exhibited a positive DAB reaction, indicative of catalase activity, which was inhibited by aminotriazole.
    • Ariolimax columbianus peroxisomes did not yield a positive DAB reaction, despite variations in the staining protocol.
    • Peroxisomes stained negatively, while lysosomes stained positively for acid-phosphatase in both species.

    Conclusions:

    • Peroxisomes are present in the kidney sac cells of Arion ater and Ariolimax columbianus.
    • Biochemical differences, likely in catalase content or accessibility, exist between the peroxisomes of these two gastropod species.
    • The DAB staining technique is a useful tool for characterizing peroxisomes, but its effectiveness can vary depending on the specific organism and tissue.

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