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Real-time two-photon confocal microscopy using a femtosecond, amplified Ti:sapphire system
G J Brakenhoff1, J Squier, T Norris
1Department of Molecular Cell Biology, University of Amsterdam, The Netherlands.
Journal of Microscopy
|March 1, 1996
Abstract:
The bilateral imaging approach known from confocal applications operating in the line mode was used to realize real-time two-photon imaging. It is shown that the sectioning inherent to two-photon imaging could be improved by the introduction of a confocal line aperture in the imaging path. Using a high-power, low-repetition-rate amplified Ti:sapphire system, various biological objects were visualized including live boar sperm.